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Metformin Prevents H₂O₂-Induced Senescence in Human Lens Epithelial B3 Cells

Chunmei Zhang, Mengmeng Chen, Nan Zhou, Yanhua Qi

(Department of Ophthalmology, Harbin Medical University, The 2nd Affiliated Hospital, Harbin, Heilongjiang, China (mainland))

Med Sci Monit Basic Res 2020; 26:e923391

DOI: 10.12659/MSMBR.923391

BACKGROUND: The primary purpose of this study was to investigate the protective effect of metformin against hydrogen peroxide (H₂O₂)-induced cellular senescence and to explore the underlying molecular mechanism of lens epithelial cell senescence.
MATERIAL AND METHODS: We used H₂O₂ to establish senescence in human lens epithelial B3 cells. The cells were exposed to H₂O₂ for different numbers of days to mimic aging. Senescence was assessed by senescence-associated ß-galactosidase staining, and the molecular mechanism was assessed by real-time polymerase chain reaction (RT-PCR) and western blot analysis. The cultured cells were exposed to 150 μM H₂O₂ for 7 days with or without metformin to detect the underlying molecular mechanism of lens epithelial cell senescence.
RESULTS: The lens epithelial cells exposed to 150 µM H₂O₂ for 7 days exhibited senescence. The expression levels of senescence-related markers were increased in H₂O₂-treated cells. Metformin prevented H₂O₂-induced cellular senescence in human lens epithelial B3 cells.
CONCLUSIONS: These findings suggest that senescence marker expression is increased in the cells exposed to H₂O₂. Metformin protects human lens epithelial B3 cells from H₂O₂-induced senescence.

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