Scimago Lab
powered by Scopus
eISSN: 2325-4416
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST


Home Page

Medical Science Monitor Basic Research has been selected for the ESCI - Emerging Sources Citation Index (Thomson Reuters, Web of Science, ISI), launching in November 2015 as a new edition of the Web of read more

Published: 2018-12-10

Curcumin-Mediated Induction of Apoptosis in Human Glioma CHME Cells

Yunqiang Zhang, Lijun Tu, Xiuhong Zhou, Bin Li

(Department of Neurosurgery, Chenzhou No. 1 People’s Hospital, Chenzhou, Hunan, China (mainland))

Med Sci Monit Basic Res 2018; 24:216-224

DOI: 10.12659/MSMBR.912313

BACKGROUND: Curcumin has clear anti-tumor activity in various carcinomas. It regulates various signaling pathways like Wnt/β-catenin and JAK2/STAT3, which play vital roles in cell proliferation of several carcinomas, but to the best of our knowledge, there are currently no published reports on human glioma CHME cells. Therefore, the aim of this study was to explore the effect of curcumin on human glioma CHME cells.
MATERIAL AND METHODS: The CHME cell line was purchased from American Type Culture Collection (ATCC). The expressions of caspases 3, caspases 9, PARP, BAX, and BCL2 were detected by Western blot. Annexin V FITC, mitochondrial membrane potential, and reactive oxygen species were detected by flow cytometry. DAPI staining was detected by fluorescence microscopy. Cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay.
RESULTS: We found that curcumin has cytotoxic activity in human glioma CHME cells, as shown by DAPI staining, annexin V/PI, and nuclear morphology. We found that cell growth decreased with increased concentration of curcumin, as well as sowing effects on expression of caspase-3, caspase-9, and cleavage of PARP, which suggests apoptotic cascade activity. The increase in reactive oxygen species and loss of mitochondrial membrane potential (Δψmt) in concentration-dependent manners suggests biochemical induction of apoptosis in CHME cells.
CONCLUSIONS: Curcumin has effective anticancer activity in human glioma CHME cells by inducing the apoptotic pathway.

Keywords: Apoptosis, Caspases, Curcumin, Glioma, Membrane Potential, Mitochondrial, Reactive Oxygen Species

Related Articles (0)

Coming Soon...

Published: 2018-12-03

Effect of Tai Chi Exercise on Balance Function of Stroke Patients: A Meta-Analysis

Shouzhi Wu, Jian Chen, Shuyi Wang, Mingfei Jiang, Ximei Wang, Yufeng Wen

Med Sci Monit Basic Res 2018; 24:210-215

DOI: 10.12659/MSMBR.911951

Published: 2018-11-26

Correlation Between First Morning Single Void and 24-Hour Urines: The Reliability to Quantify Niacin Stat...

Luiz Ricardo Soldi, André Luiz Maltos, Daniel Ferreira da Cunha, Guilherme Vannucchi Portari

Med Sci Monit Basic Res 2018; 24:206-209

DOI: 10.12659/MSMBR.910087