14 April 2017 : Animal Research
High-Performance Liquid Chromatography (HPLC) Quantification of Liposome-Delivered Doxorubicin in Arthritic Joints of Collagen-Induced Arthritis Rats
Hongqing Niu1ABEF, Menghua Xu1ABC, Shuangtian Li1BF, Junwei Chen1AD, Jing Luo1CG, Xiangcong Zhao1BC, Chong Gao2AF, Xiaofeng Li1AEG*DOI: 10.12659/MSMBR.904103
Med Sci Monit Basic Res 2017; 23:150-158
Abstract
BACKGROUND: Neoangiogenesis occurring in inflamed articular synovium in early rheumatoid arthritis (RA) is characterized by enhanced vascular permeability that allows nanoparticle agents, including liposomes, to deliver encapsulated drugs to arthritic joints and subsequently improve therapeutic efficacy and reduce adverse effects. However, the targeting distribution of liposomes in arthritic joints during RA has not been quantitatively demonstrated. We performed this study to evaluate the targeting distribution of PEGylated doxorubicin liposomes in the arthritic joints of collagen-induced arthritis (CIA) rats by high-performance liquid chromatography (HPLC).
MATERIAL AND METHODS: Two doxorubicin formulations were administered to CIA rats via tail intravenous injection at a single dose (50 mg/m²). CIA rats were sacrificed and the tissues of the inflamed ankle joints were collected. The content of doxorubicin in the arthritic joints was analyzed by a validated and reproducible HPLC method. A two-way ANOVA for 2×5 factorial design was used for statistical analysis.
RESULTS: The developed HPLC method was sensitive, precise, and reproducible. The method was successfully applied to quantify doxorubicin content in arthritic tissues. At each time point (6, 12, 24, 48, and 72 h), doxorubicin content in the arthritic joints of the doxorubicin liposome group (DOX-LIP group) was higher than in the free doxorubicin group (DOX group) (P<0.05). In the DOX-LIP group, doxorubicin levels in the arthritic joints increased gradually and significantly in the interval of 6–72 h post-administration.
CONCLUSIONS: PEGylated doxorubicin liposomes were targeted to, accumulated, and retained in the arthritic joints of CIA rats. The present study indicates that liposome encapsulation increases the therapeutic efficacy of antirheumatic drugs, presenting a promising therapeutic strategy for RA.
Keywords: Arthritis, Experimental, Arthritis, Rheumatoid, Chromatography, High Pressure Liquid, Drug Delivery Systems, Liposomes
Most Viewed Current Articles
30 Oct 2023 : Original article 5,615
Exploring the Impact of the COVID-19 Pandemic on Academic Burnout Among Nursing College Students in China: ...DOI :10.12659/MSMBR.940997
Med Sci Monit Basic Res 2023; 29:e940997
22 Mar 2023 : Clinical Research 4,509
A Questionnaire-Based Study to Compare the Psychological Effects of 6 Weeks of Exercise in 123 Chinese Coll...DOI :10.12659/MSMBR.939096
Med Sci Monit Basic Res 2023; 29:e939096
10 Jan 2023 : Clinical Research 3,531
Prevalence and Associated Factors of Depression Among Frontline Nurses in Wuhan 6 Months After the Outbreak...DOI :10.12659/MSMBR.938633
Med Sci Monit Basic Res 2023; 29:e938633
06 Nov 2023 : Original article 3,125
Urinary Klotho Excretion: A Key Regulator of Sodium Homeostasis in Chronic Kidney Disease Stage 2-4DOI :10.12659/MSMBR.942097
Med Sci Monit Basic Res 2023; 29:e942097