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Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

Vesara A. Gatera, Ronny Lesmana, Ida Musfiroh, Raden Tina D. Judistiani, Budi Setiabudiawan, Rizky Abdulah ORCID logo

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia

Med Sci Monit Basic Res 2021; 27:e931481

DOI: 10.12659/MSMBR.931481

Available online: 2021-06-09

Published: 2021-06-09


BACKGROUND: Studies have shown that lung inflammation affects lung function, with life-threatening results. Vitamin D may play an important role in inhibiting inflammatory cytokines. Vitamin D deficiency is related to several lung problems, including respiratory distress syndrome, alveolar inflammation, epithelial damage, and hypoxia. Few studies have evaluated the benefits of vitamin D in preventing inflammation in alveolar cells.
MATERIAL AND METHODS: We developed a cell inflammation model induced by lipopolysaccharide (LPS) treatment. The effects of vitamin D on LPS-induced inflammation in A549 cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anti-inflammatory mechanism of vitamin D was evaluated using western blot analysis.
RESULTS: Our results indicated that vitamin D promoted A549 cell survival following LPS-induced inflammation by downregulating nuclear factor nuclear factor kappa light chain enhancer of activated B cells, tumor necrosis factor-alpha, interleukin (IL)-1ß, IL-6, and IL-12.
CONCLUSIONS: Our results indicated that vitamin D has the potential to manage lung inflammation, although further studies are needed.

Keywords: 1,25-dihydroxyvitamin d, Cytokines, Inflammation, Lipopolysaccharides, Lung